Plants are traditional sources of several bioactive compounds like alkaloids, flavonoids, tannins, saponins, terpenes and phenolic compounds. In this study, three different clones of Hevea brasiliensis, namely RRII 105, RRII 414 and RRII 430, were selected and subjected to successive solvent extraction using methanol, isopropanol, acetone and cyclohexane, followed by GC-MS/MS analysis for the identification of various bioactive compounds. The chromatogram was recorded and matched with the NIST library for identification of compounds. Qualitative assays confirmed the presence of alkaloids, flavonoids, phenolics, tannins, saponins and terpenoids across all clones. Extraction efficiency differed among solvents, with methanol consistently yielding the highest recovery of compounds. Notable compounds identified included friedelinol, ẞ-D- glucosyloxyazoxymethane, loliolide, para-vinylguaiacol and 2,3-dihydrobenzofuran, which are associated with antioxidant, anti-inflammatory, antimicrobial, anticancer and biopolymer precursor activities. These findings establish Hevea leaves as a promising source of bioactive secondary metabolites and the use of these compounds in various fields needs to be explored, especially in drug development and to gain an understanding of the metabolic pathways in plants.
Bioactive compounds; Clonal variation; GC-MS/MS, Hevea brasiliensis; Phytochemicals; Solvent extraction