A process was developed for the preparation of high quality solid low protein natural rubber (LPNR-S) which could be scaled up to larger volumes of industrial level. In the new process,creaming or centrifuging of the latex employed in many of the deproteinization process were avoided. The ammoniated field latex was treated with a deproteinization mixture developed at Rubber Research Institute of India. The treated latex was coagulated after specified time with formic acid. The coagulum was creeped, washed and dried to get solid LPNR. The study showed that LPNR-S with nitrogen content of 0.07 to 0.1 per cent could be prepared from low ammonia latex within 24 h of deproteinization time. The same low nitrogen content could be achieved in LPNR-S within one hour of deproteinization time, using high ammonia latex and varying the composition of the deproteinization mixture.The optimized method reduces the protein content of LPNR-S by 85 per cent to that of the control. The LPNR-S prepared by new method had better raw rubber properties than commercial DPNR samples. The LPNR-S samples prepared through different methods were used to make corresponding LPNR-S composites. The impact of protein removal on technological properties and ageing behaviour of the LPNR-S composites at low and high antioxidant levels was evaluated.
A simple, accurate and quick method for determining DRC in fresh and ammoniated field latex was developed and standardised. The new procedure uses ethyl alcohol (70%) or Medispirit (70% isopropyl alcohol) as the coagulant. To about 15 ml of the coagulant taken in a petri dish, approximately 10g of fresh field latex was added drop by drop and swirled till it coagulated into a mass. The instantly formed coagulum was pressed using a flat glass stopper. After washing and rolling, the thin and tender coagulum required only 90-120 min. drying at 80oC for obtaining constant weight. On the contrary, the standard procedure for DRC estimation using dilute acetic acid as coagulant took 1-2 h for coagulum formation and about 16 h for drying to constant weight. The easy instant coagulation of field latex using the new alcohol coagulant is the novelty of the method and can be easily adopted in the field. Hence, this new method is very useful for the estimation of dry rubber yield of experimental plants in the field in the clone evaluation trials or in other field experiments requiring yield assessment as well as in Group Processing Centres etc.